Immunohistochemical staining of RANKL in formalin-fixed, paraffin-embedded human lymph node using R1075-13.

Immunohistochemistry-Paraffin: formalin-fixed, paraffin-embedded human liver stained with R1075-13 (1:500, 1ug/ml), peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.

Immunohistochemistry-Paraffin: formalin-fixed, paraffin-embedded human lymph node probed with R1075-13 (5ug/ml). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.